How do you quantify reliability for your mass cytometry standard panel on whole blood samples?

We completed rigorous tests as part of our CLIA registration process. We analyzed whole blood from 6 healthy donors after processing with our TokuKit-SH, and looked at 6 measures. For each measure, we set a coefficient of variation (CV) threshold, based on the literature. As a refresher, the coefficient of variation is simply the standard deviation divided by the mean. In general, a smaller CV is better.

• Intra-run: Same sample, different runs
  • 2.16% versus CV threshold of 25% criteria
  • 3 fixed whole blood samples were divided into 3 replicates and run on CyTOF on the same day
• Inter-run: Same sample, same run
  • 6.83% versus 30% CV criteria
  • 3 replicates from 3 fixed whole blood samples were run on different days
• Inter-instrument: Same sample, different instrument
  • -1.28% versus 25% CV criteria
  • 2 replicates from 3 fixed whole blood samples were run on different instruments
• Inter-kit: Same sample, different TokuKit
  • 5.76% versus 25% CV criteria
  • 3 whole blood samples processed by 3 different TokuKits each were compared
• Pre-Fixation Stability: Same unfixed sample, across time
  • 0.12% versus +/- 25% change between baseline and 2 hours later
  • 3 whole blood samples were split, stored at 4C, and processed at different timepoints
• Post-Fixation Stability: Same fixed sample, across time
  • 3.61% versus +/- 25% change between baseline and 1 month later
  • 3 whole blood samples were processed with the TokuKit, split, stored at -80C, and processed at different timepoints

Want to read more?

Our full validation report also includes a description of all protocols and methods, assay design including step-by-step gating strategy, and more.

Read the full report here