Feature | TokuKit Whole Blood Collection | PBMC Isolation |
---|---|---|
Processing time per sample | 20 min | 1.5 hrs |
Include granulocytes | Yes | No |
Cell viability | >95% | ~85% |
Special equipment needed | No | Yes (centrifuge, Ficoll, etc.) |
Cost | $45 | $200-300 |
Whole Blood Collection
Our TokuKits are designed to eliminate the need for PBMC isolationPBMCs or Whole Blood?
With whole blood you get the same quality information about non-granulocytes as with PBMCs, plus additional insights into granulocyte populations (neutrophils, eosinophils, basophils).
CLIA Validation Data
Mass Cytometry Assay for Pan-Immune Profiling of Whole Blood Collected with TokuKit-SHFill out the form below to access our CLIA Validation plan, report, data and SOPs.
Want to order TokuKits for your clinical trial site?
Here’s how to place an orderAll kits will be charged to a sponsor account.
Once you click the link below, you can get started with ordering.
- Set up cart screen
- Specify the quantity of kits you want. Then click “add to cart” and go to the next page.
- Review cart screen
- Now, enter your NCTID. If you don’t have one, just enter NCT000000000
- Site ID: if you don’t have one, you can leave this blank.
- Order special instructions: Note any special handling instructions in this field. Then click “check out.”
- Check out screen
- Enter the shipping email and contact information.
- For billing address select “Same as shipping.” The study sponsor will receive the invoice for this purchase.
Collection
Collect whole blood into the provided vacutainer.
Step One: RBC Lysis & Fixation
Add two mL of blood to Buffer 1, mix, and leave at room temperature for 15 min.
Step Two: Quench & Stabilize
If using SH or SE kits, add full volume to Buffer 2 and mix to complete sample preparation.
Storage & Shipping
Store short-term at -20°C or long-term at -80°C. Ship overnight on dry ice.