Spectral Flow Cytometry
We analyze 153 subsets and functional states across millions of cells using spectral flow cytometrySpectral flow cytometry is a technology used to determine specific properties of cells.
It is an advanced cytometry method that builds upon the established science of flow cytometry while eliminating many of the panel design limitations associated with the technique.
Armed with in-depth immune information, researchers can make informed decisions around patient response and drive new pathway discoveries.
Conventional Flow Cytometry
- 10-15 markers per assay
- Significant signal overlap into nearby detectors requires manual compensation
- Multiple sample consumption for analysis of multiple cell types
- Marker redundancy across panels reduces usable data per assay
Spectral Flow Cytometry
- 25-40 markers per assay
- Full spectrum detection and unmixing resolves spectral overlap
- Single sample consumption for analysis of all major immune cell subsets
- Single panel limits redundancy, providing more data per assay
Profile 150+ immune subsets and functional states
Teiko Bio’s Validated 25-Marker Spectral Flow Pan-Immune Profiling Test for Human PBMCs includes:
- All major innate and adaptive immune cell populations
- 6 functional state markers of maturation, activation, and exhaustion
- State marker expression as median fluorescence intensity (MFI)
25-Marker Human PBMC Pan-Immune Profiling Panel
Teiko Bio’s 25-Marker Spectral Flow Pan-Immune Profiling Test includes:
- Markers to identify every major immune cell population and subsets
- 6 functional state markers of maturation, activation, and exhaustion
- Gating performed by our team of expert immunologists
-
T cells
- CD3
- Total T cells
- CD4
- CD4+ T cells
- CD8
- CD8+ T cells
- CD25
- Treg, activation
- CD127
- T cell subsets
- CCR7
- Naive/Memory
- CD45RA
- Naive/Memory
- CD27
- Maturation
- CD28
- Maturation
- TCRgd
- gdT cells
- CD56
- NKT cells
- CD38
- Activation
- HLA-DR
- Activation
- PD-1
- Exhaustion
-
B cells
- CD19
- Total B cells
- CD20
- B cell subsets
- CD27
- Naive/Memory
- IgD
- B cell subsets
- IgM
- B cell subsets
- CD25
- Activation
- HLA-DR
- Antigen presentation
-
NK cells
- CD56
- NK cell subsets
- CD16
- NK cell subsets
- CD38
- Activation
-
Myeloid cells
- CD14
- Monocyte subsets
- CD16
- Monocyte subsets
- CD11c
- Monocytes, macrophages, DCs
- CD123
- pDC
- CD141
- cDC1
- CD1C
- cDC2
- HLA-DR
- Antigen presentation
-
Granulocytes
- CD123
- Basophil
-
General
- CD45
- Total immune cells
- ViaDye
- Viability
CLIA Validation Data
25-Marker Spectral Flow Pan-Immune Profiling Test for Human PBMCsFill out the form below to access our CLIA Validation plan, report, and data and SOPs.
Standard 25-Marker TokuProfile
Includes 25 markers
Based on our CLIA-validated 25-marker Peripheral Blood Mononuclear Cell (PBMC) panel.
10 sample minimum
10 sample minimum per project; minimum of 10 samples per batch for larger projects.
Special introductory pricing
Take advantage of our special introductory pricing for early adopters
Basic
Includes
- Gated Data
- .FCS and .gatingml Files
- 1 Consulting Hour
- 1 week turnaround time
Core
Includes
- Gated Data
- .FCS and .gatingml Files
- Gated Analysis Results
- Multi and Two-Group Statistical Testing, PCA
- Web App
- Composition, Box and Time Series Plots
- 2 Consulting Hours
- 2.5 weeks turnaround time
Premier
Includes
- Gated Data
- .FCS and .gatingml Files
- Gated Analysis Results
- Multi and Two-Group Statistical Testing, PCA
- Web App
- Composition, Box and Time Series Plots
- Unsupervised Analysis Results
- FastPG Clustering, UMAP, PCA, Statistical Testing
- 2 Consulting Hours
- Coming Q1:24