TokuKit Stabilization Protocol

Collect Blood

Collect Blood

Collect blood at clinical site & invert the vacutainer 10x.

Lyse & Fix

Lyse & Fix

Transfer 2 mL of blood to Buffer 1; Incubate at room temp for 15 mins.

Stabilize & Store

Stabilize & Store

Transfer entire volume to Buffer 2 and mix. Store in -80C or ship on dry ice.

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Mass Cytometry

0.99+ Correlation

Between immune cell population frequencies in live and fixed whole blood on mass cytometry.
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5 hours to process a sample, 24 months to analyze

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Spectral Flow Cytometry

0.97+ correlation

Between immune cell population frequencies in live and fixed whole blood on spectral flow cytometry.
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