Whitepaper: 25-Marker Spectral Flow Panel for Immune Profiling in Fixed Whole Blood

Whitepaper highlights:

• In this study, we compared live whole blood to whole blood stabilized using Teiko’s TokuKit and evaluated the stability of a 25-marker spectral flow panel under various precision tests, such as inter- and intra-run.
• The panel showed a high correlation (r=0.97) between live and fixed samples when measuring phenotypic immune cell frequencies.
  • For intra-run and inter-run precision tests median coefficients of variation (CV) were well under 10% for most cell populations.
• Fixed whole blood samples stored at -80°C remained stable for up to 24 months (accelerated), with more than 90% of populations exhibiting less than a 25% change from baseline.
• These results demonstrate that TokuKit fixation reliably preserves immune cell populations over extended periods, reducing the logistical challenges of live sample handling in large or multi-centered clinical trials and ensuring high-quality, reproducible data for immunophenotyping studies.

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