Yes, conditionally, and no.
Yes – Transcription factors and other highly expressed intracellular markers can easily be detected. Our base panels typically include transcription factors such as Foxp3, T-bet, and TCF1 and intracellular functional proteins like Granzyme B and KI-67. We have also validated a number of other transcription factors like HIF1a and RORgt, intracellular proteins like Perforin and X, and even metabolic markers like mTOR and GAPDH.
Conditionally – Some cytokines, such as IFNg and TNFa, can be detected in activated cells without additional stimulation. However, most cytokines typically require cell stimulation in the presence of a secretion blocker (like Brefeldin A) in order to build up sufficient protein for detection. Our scientists can help you determine whether prior stimulation may be necessary depending on your cytokine of interest.
No – Chemokines are often produced in low quantities not well-suited for cytometry based detection. While chemokine production cannot be easily detected, chemokine receptor expression is detectable and can provide information about which immune subsets are responsive to chemokine gradients. In-house validated antibodies