Can you work with any antibody / stain any marker? Even our own custom antibody?

Yes, we can work with any purified antibody that is stored in a protein-free format (i.e. without BSA or other added proteins). For surface markers, we can work with both IgG and IgM antibodies. For intracellular markers, we recommend using IgG antibodies as they tend to better penetrate into the cell. We do not work with Fab fragments due to incompatibility with our conjugation procedure.

Metal conjugation is performed in-house and the newly metal-conjugated antibody is tested and optimized on in-house PBMC samples. If your marker of interest is not expressed on PBMCs, a cell line or other sample type should be provided or can be purchased for panel validation purposes.