Can blood fixed with your TokuKits be used for conventional flow?

Maybe, but we have not tested the use of TokuKits for conventional (non-spectral) flow cytometry internally yet. However, two papers published in peer-reviewed journals have shown that it is possible to fix blood using the Stable-Lyse/Stable-Store (Nguyen et al.) and PROT1 (Silva et al.) buffers that we use in our TokuKits and run the samples on conventional flow. These papers found that some markers are not impacted by fixation, while others are, meaning that panels need to be created carefully.

To explore the feasibility of using these buffers for spectral flow cytometry, Teiko is currently testing our 25-marker spectral flow cytometry panel on whole blood samples fixed with our Stable-Lyse/Stable-Store TokuKits. The results will be posted on our website in Q2:24.