For prospective studies, we recommend collecting whole blood into standard vacutainers containing, in order of preference, heparin, citrate, or EDTA and using our simple two-step, 20 minute TokuKit for on-site processing. This process is faster, easier, and cheaper than PBMC isolation, has no centrifugation steps, and yields high-quality cytometry data. Blood Collection Guidelines
For retrospective studies, we accept viably cryopreserved PBMCs or whole blood samples that have undergone red blood cell lysis and fixation using PROT1, or Stable-Lyse, Stable-Store.
We cannot accept whole blood collected using an RNA or DNA isolation tube, nor serum samples as these lack intact cells required for cytometry-based analyses.