Do you just do cookie cutter gating?

Short version: no. We are gluttons for gating punishment here at Teiko. We have dozens of quality control checks to make sure that your gating reflects biological reality. Let’s take a tour.

Custom panels: We work out a gating strategy together with you to ensure that all populations of interest are analyzed. Once finalized, we present the gating strategy on a control sample for you to review and approve. If you have changes to make, we can easily incorporate them at this stage.

Standard panels: We review our standard gating scheme with you and you have the ability to make adjustments. This review process ensures that you get correctly gated data.

Post-processing: After samples are processed, we apply your approved gating strategy. We carefully review and adjust (also known as “tailor”) each plot within a sample. This means that we individually adjust thousands of plots per project. After thousands of samples processed, our scientists know when to apply cutoffs. For example, if two patients have different levels of a certain marker, we would need to adjust a gate cutoff. However, if we’re looking at a time-series of an individual, we might need to apply the same cutoff to multiple specimens of that patient to detect changes over time.

This is a crucial step. If we didn’t do this kind of thorough inspection, you would end up with cookie cutter gating, junk data, and ultimately incorrect population measurements.

Independent review: We don’t stop there. Since we like pain, we have another scientist independently review every single plot to make sure nothing gates are placed correctly and nothing gets missed.

See for yourself: After gating, but before we put the data on your dashboard, we present you with compact, letter-size overviews showing the complete gating tree for every sample. This way you can quickly and easily review all gates of an entire sample.

At this point, you have three calendar days to review the gating placement, and request adjustments of gates. This gives you complete visibility into gating and ensures there are no surprises.

Our method gives you total visibility into how your samples are being analyzed at every step of the way. No more rigid reports where you have to spend weeks repairing gating from scratch.

Questions about our gating? Check out free demo datasets at app.teiko.bio.