Heading to the International Clinical Cytometry Conference (ICCS) in Seattle this October? Meet us there!
Join us on Monday, October 7, at 4:00 PM for the presentation of two posters assessing normalization methods for CyTOF data and analyzing fixed whole blood samples by high-dimensional spectral cytometry.
Abstract #205: Assessment of Batch Normalization Methods on Mass Cytometry Data
Presenter: Dr. Gage Black, PhD, Teiko Bio
Batch effects can impact the quality of large biological datasets, including high-dimensional mass cytometry data. In this study, we tested two batch normalization tools, CytoNorm and cyCombine, on a dataset of 68 PBMC samples from 38 melanoma patients.
Abstract #226: Rapid on-site fixation of whole blood samples for high-dimensional spectral flow cytometric analysis
Presenter: Dr. Justin Jarrell, PhD, Teiko Bio
Around 75% of flow cytometry specimens rely on freshly collected blood, requiring processing within 48 hours. This causes complex logistics and can result in >10% sample loss. Fixing whole blood at clinical sites may solve this by preserving samples for years. We used a 25-marker spectral flow cytometry panel on blood from three healthy donors, comparing fixed to live samples. Fixed samples showed a 97% correlation in immune cell populations and a 90% correlation for activation markers (HLA-DR, CD28, CD38, PD-1). Fixation simplifies shipping while preserving sample integrity for clinical trials.
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