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Assessment of Batch Normalization Methods on Mass Cytometry Data

Chronister, Leavitt, & Black, et al. ICCS (2024)
Assessment of batch normalization methods on mass cytometry data
Assessment of batch normalization methods on mass cytometry data

Poster Highlights

  • In this study, we used 68 PBMC samples from melanoma patients to assess the effectiveness of two batch normalization tools, CytoNorm and cyCombine.
  • We found that both methods preserved biological variance, through the evaluation of their MAD scores.
  • CyCombine demonstrated superior performance in reducing technical variation with an Earth Mover’s Distance reduction of 0.70, compared to CytoNorm’s 0.37.
  • The findings highlight the complexity of removing batch variation without distorting true biological signals, emphasizing the need for careful selection of normalization methods in multi-batch experiments.

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Reference citation: Chronister WD, Leavitt LS, Black GS, et al. Assessment of batch normalization methods on mass cytometry data. 2024. The 39th International Clinical Cytometry Society Meeting.