Whole Blood Preservation for Clinical Trials

TokuKit

Preserve immune cell populations and functional marker subsets for months.

 The three step, centrifuge-free process used at over 40 global trial sites.

3-Step Preservation

Collect, Fix, and Store

Collect Blood

Collect at least 2 mL of blood into a 4 mL vacutainer at the clinical site and invert 10x.

Fix Sample

Transfer 2 mL of blood to Buffer 1; incubate at room temp for 15 mins.

Store in Freezer

Transfer 4.8 mL of sample to Buffer 2; store at -80°C or ship on dry ice.

TokuKit Stability Data

Mass Cytometry

Preservation data on our 41-Marker Standard Panel
15 Month Stability
Preserve population frequencies and protein expression levels from blood draw for 15 months (and counting).
  • R > 0.99
    Correlation of population frequencies
  • R = 0.96
    Correlation of functional marker subsets
  • R = 0.95
    Correlation of protein expression

Post-fixation Stability

Fixed like fresh by mass cytometry
Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.
  • R > 0.99
    Correlation of population frequencies
  • R = 0.93
    Correlation of functional marker subsets
  • R = 0.85
    Correlation of protein expression

Fresh versus TokuKit-Fixed Specimens

TokuKit Stability Data

Spectral Flow
Cytometry

Preservation data on our 25-Marker Standard Panel
6 Month Stability
Preserve population frequencies and protein expression levels from blood draw for 6 months (and counting).
  • R > 0.99
    Correlation of population frequencies
  • R = 0.98
    Correlation of functional marker subsets
  • R = 0.86
    Correlation of median fluorescence intensity

Post-fixation Stability

Fixed like fresh by spectral flow
Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.
  • R = 0.97
    Correlation of population frequencies
  • R = 0.91
    Correlation of functional marker subsets
  • R = 0.84
    Correlation of median fluorescence intensity

Fresh versus TokuKit-Fixed Specimens

TokuKit Validated Antibody Clones

Which antibody clones are compatible with TokuKit?

Teiko has performed a titration for each antibody clone on this list and determined an optimal staining concentration, based on staining index, arcsinh ratio, positive/negative separation, and spillover into neighboring channels.
TokuKit Pre-Fixation Stability Data

Can't immediately process samples with TokuKit?

If necessary, fresh whole blood samples can be kept at room temperature for up to 9 hours in EDTA vacutainers, or up to 5 hours in Sodium Heparin vacutainers, before processing with TokuKit.

Pre-Fixation Stability Data

TokuKit Stability Data at 4°C

Don't have access to a -80°C freezer?

If necessary, TokuKit-fixed samples can be stored at 4°C for up to 14 days before transferring to a -80°C freezer.

TokuKit-Fixed Specimens, Storage at -80°C versus 4°C 

TokuKit

Interested in documentation?

Instruction manuals, safety data sheets, and regulatory documentation are available upon request.
Contact us today
See TokuKit in Action

Check our TokuKit demo

Using TokuKit

Can clinical sites actually do this?

3000+ TokuKits Shipped

US, EU, Australia, China sites

Only Personal Protective Equipment needed

Work with Central Labs (ICON, PPD, LabCorp, etc.)

"This is [like] the other samples that are being handled per Lab Manual ie PK samples, ADA samples etc,where tube is spun and serum transferred out and aliquoted"

-Translational scientist, publicly traded immunotherapy company