Whole Blood Preservation for Clinical Trials

TokuKit

Preserve immune cell populations and functional marker subsets for months.

 The three step, centrifuge-free process used at over 40 global trial sites.

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3-Step Preservation

Collect, Fix, and Store

Collect Blood

Collect at least 2 mL of blood into a 4 mL vacutainer at the clinical site and invert 10x.

Fix Sample

Transfer 2 mL of blood to Buffer 1; incubate at room temp for 15 mins.

Store in Freezer

Transfer 4.8 mL of sample to Buffer 2; store at -80°C or ship on dry ice.

TokuKit Stability Data

Mass Cytometry

Preservation data on our 41-Marker Standard Panel
15 Month Stability
Preserve population frequencies and protein expression levels from blood draw for 15 months (and counting).
  • R > 0.99
    Correlation of population frequencies
  • R = 0.96
    Correlation of functional marker subsets
  • R = 0.95
    Correlation of protein expression

Post-fixation Stability

Cell Populations
Functional Marker Subsets
Marker Expression
1 Month
3 Month
9 Month
15 Month
1 Month
3 Month
9 Month
15 Month
1 Month
3 Month
9 Month
15 Month
Fixed like fresh by mass cytometry
Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.
  • R > 0.99
    Correlation of population frequencies
  • R = 0.93
    Correlation of functional marker subsets
  • R = 0.85
    Correlation of protein expression

Fresh versus TokuKit-Fixed Specimens

Cell Populations
Functional Marker Subsets
Marker Expression
TokuKit Stability Data

Spectral Flow
Cytometry

Preservation data on our 25-Marker Standard Panel
6 Month Stability
Preserve population frequencies and protein expression levels from blood draw for 6 months (and counting).
  • R > 0.99
    Correlation of population frequencies
  • R = 0.98
    Correlation of functional marker subsets
  • R = 0.86
    Correlation of median fluorescence intensity

Post-fixation Stability

Cell Populations
Functional Marker Subsets
Marker Expression
1 Month
4 Month
6 Month
1 Month
4 Month
6 Month
1 Month
4 Month
6 Month
Fixed like fresh by spectral flow
Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.
  • R = 0.97
    Correlation of population frequencies
  • R = 0.91
    Correlation of functional marker subsets
  • R = 0.84
    Correlation of median fluorescence intensity

Fresh versus TokuKit-Fixed Specimens

Cell Populations
Functional Marker Subsets
Marker Expression
TokuKit Validated Antibody Clones

Which antibody clones are compatible with TokuKit?

Teiko has performed a titration for each antibody clone on this list and determined an optimal staining concentration, based on staining index, arcsinh ratio, positive/negative separation, and spillover into neighboring channels.

Can TokuKit measure absolute cell counts?

Yes, Teiko integrates complete blood counts from your central lab with cytometry data from TokuKit-fixed samples to calculate absolute cell counts.

Read about our full workflow here
1
Collect two aliquots of blood

One sample is preserved with TokuKit for cytometry, while the other is kept fresh for hemoanalysis.

2
Process aliquots separately

The fresh sample is sent to your central lab for hemoanalysis to measure leukocytes per µL, while the TokuKit-fixed sample is sent to our lab for cytometry.

3
We integrate the data

Our computational pipeline derives absolute cell counts by multiplying population frequencies with the leukocytes per µL value, then displays the results on the dashboard.

TokuKit Pre-Fixation Stability Data

Can't immediately process samples with TokuKit?

If necessary, fresh whole blood samples can be kept at room temperature for up to 9 hours in EDTA vacutainers, or up to 5 hours in Sodium Heparin vacutainers, before processing with TokuKit.

Pre-Fixation Stability Data

EDTA Vacutainer
Sodium Heparin Vacutainer
TokuKit Stability Data at 4°C

Don't have access to a -80°C freezer?

If necessary, TokuKit-fixed samples can be stored at 4°C for up to 14 days before transferring to a -80°C freezer.

TokuKit-Fixed Specimens, Storage at -80°C versus 4°C 

Cell Populations
Functional Marker Subsets
Marker Expression
TokuKit

Documentation

Instruction manuals, safety data sheets, and regulatory documentation.
Product Specifications
TokuKit Processing Instructions
Reagent Safety Data Sheets
Regulatory Documentation
Product Specifications
Each TokuKit contains the following:
  • One 4-mL vacutainer
  • Two disposable transfer pipettes
  • One 15-mL red top nalgene bottle with 2.8 mL of Stable Lyse buffer
  • One 15-mL blue top nalgene bottle with 8 mL of Stable Store buffer
  • One liquid absorbent pad
  • One resealable plastic biohazard bag
Product dimensions: 6"x4"x2" in
TokuKit Processing Instructions
Reagant Safety Data Sheets
See TokuKit in Action

Check our TokuKit demo

Using TokuKit

Can clinical sites actually do this?

2000+ TokuKits Shipped

US, EU, Australia, China sites

Only Personal Protective Equipment needed

Work with Central Labs (ICON, PPD, LabCorp, etc.)

"This is [like] the other samples that are being handled per Lab Manual ie PK samples, ADA samples etc,where tube is spun and serum transferred out and aliquoted"

-Translational scientist, publicly traded immunotherapy company

Teiko

Cytometry made simple for global clinical trials.
Spectral Flow: Whole Blood PanelMass Cytometry: Whole Blood PanelWhole Blood Stabilization: TokuKitDashboards & ReportsGating
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