Rapid on-site fixation of whole blood samples for high-dimensional spectral flow cytometric analysis

Poster Highlights

• In this study, we compared the differences between fresh whole blood analysis versus rapid on-site fixation, utilizing Teiko's TokuKit. Whole blood from three healthy donors was divided into two aliquots -- one for live processing, and the other for fixation.
• The assay used was adapted from the Cytek 25-color Immunoprofiling Assay, designed to assess population frequencies of immune cell subsets and function marker positivity across both the fixed and fresh samples.
• The correlation between population frequencies measured in live versus fixed samples was 0.97, and the correlation for functional marker positivity was r = 0.91. The assay exhibited an intra-run precision of 4.76% and inter-run precision at 6.89%, demonstrating the reliability of the fixation method for high-dimensional analysis.
• The findings indicate that fixation of whole blood samples preserves critical cellular characteristics an allows for identification of immune cell populations, without the limitation of a narrow analysis window.