TokuFocus: Announcing “focused” panels for spectral flow and mass cytometry

TokuFocus: Announcing “focused” panels for spectral flow and mass cytometry.

Codename: Daiki | 大喜 | Champion Sumo Wrestler

Teiko’s cytometry services allows you to measure 146 – 840 of populations, a 6-17X increase compared to conventional 13-marker flow cytometry panels.

However, many immunotherapy developers are worried about the cost and need of measuring the extra populations. Drug developers sometimes say, “I just need to measure TBNK by flow first. If the drug shows activity in the TBNK populations, then, and only then, would I care about the other populations.”

The TokuFocus is designed with this drug developer in mind. You can start with a TBNK panel subset, covering 10 markers at $1,000 per specimen. You can then upgrade to the full panel at any time within a year for an additional $900 per specimen. In short, you get the option to see the rest of the immune tree when you want (and only when you want).

What problem TokuFocus solves:

• Drug developers who need a quick analysis of TBNK populations but who don’t want to lose the option of detailed immune population analysis. These developers want to focus on TBNK populations first and analyze the rest of the immune tree after achieving successful readings.

How it works:

• Each sample will be run on a full, 25-marker or 41-marker panel.
• For $1,000 per specimen, customers can access the data for a basic 10-marker TBNK panel. 
• With the software-based upgrade, customers can unlock access to the data for the full panel for $900 per specimen, without having to run additional samples. 
• Required minimum specimens by cohort
  • Mass Cytometry: 20 specimen
  • Spectral Flow Cytometry: 50 specimen

What do I get after I upgrade?

• Gated Data
• FCS and GatingML Files
• Dashboard: Immune Cell Composition, Box and Time Series Plots
• 2 Consulting Hours

Key Features: 

With the 10-marker panel, customers can access features included in our core analysis tier. This includes data tables, sample and gating QC, and manually gated statistical analysis. Raw data files, like FCS and GatingML are only available after unlocking the remaining channels. These features will be available on Teiko’s dashboard, at app.teiko.bio. 

Within a year, customers can upgrade to unlock the data for the full panel. After upgrading, the analyzed data will be available within the dashboard, assuming a 50 specimen order.

FAQ:

• Can I do this on a custom panel?
  • Not yet. This is built for our off-the-shelf panel.
• Can I pick the subset?
  • Not yet. Here are the markers included in our basic TBNK panels:

Spectral				Mass Cytometry
PBMC (Viability, FSC x SSC)	Marker	Whole Blood (fixed)		PBMC (viability)	Marker	Whole Blood (fixed)
CD4	CD4 T cell	CD4		CD4	CD4 T cell	CD4
CD8	CD8 T cell	CD8		CD8	CD8 T cell	CD8
CD45	Leukocyte	CD45		CD45	Leukocyte	CD45
	Neutrophil Cleanup	CD66b		CD66b	Neutrophil Cleanup	CD66b
CD3	T cell	CD3		CD3	T cell	CD3
CD19	B cell	CD19		CD19	B cell	CD19
CD56	NK Cell	CD56		CD56	NK Cell	CD56
CD16	NK, Monocyte	CD16		CD16	NK, Monocyte	CD16
CD14	Monocyte	CD14		CD14	Monocyte	CD14
CD11c	Conventional DC	CD11c		CD11c	Conventional DC	CD11c

• What if I want to run multiple cohorts?
  • We can support this, however spectral flow requires 50 specimens per cohort, and mass cytometry requires 20 specimens per cohort.
• Is the full gating tree available?
  • Yes, you will get the full gating tree on the dashboard. You’ll see the unavailable populations greyed out.
• Can I cherry pick which samples to upgrade? That is, if I send you 50 samples, can I upgrade 30?
  • Not yet, you cannot select which samples to upgrade. That is, the upgrades are “all or nothing.”
• How do you do unsupervised analysis?
  • We don’t do unsupervised analysis on the focused panel. If you’re interested in that, you have to unlock the remainder of the panel. Once you unlock the panel, you have the option to upgrade for an additional fee.