News

Announcing the availability of 25 marker spectral flow cytometry assay on fixed whole blood

Published by Teiko 12/06/2024

Release: Hakuhō | 白鵬 | Champion Sumo Wrestler

We are thrilled to unveil our latest innovation for drug developers: a spectral flow cytometry panel using fixed whole blood collected with our TokuKit. This cutting-edge service allows you to analyze 146 immune populations with precision in our CLIA-registered lab.

Precision Assay	Inter-Run	Intra-Run
Benchmark	≤25%	≤20%
Median % CV Phenotypic Cell Populations	4.56%	3.38%
% of population passing	95.5%	98.2%
Median % CV Functional Marker Subsets	7.28%	5.50%
% of population passing	90.9%	93.5%

This table showcases the precision of the assay, demonstrating high reproducibility with low median % CV values for both inter-run and intra-run measurements of phenotypic cell populations and functional marker subsets.

Stability Assay	1 month	12 months (accelerated)	24 months (accelerated)
Benchmark	≤25%	≤25%	≤25%
Median % change Phenotypic Cell Populations	6.51%	5.20%	7.74%
% of population passing	90.4%	87.7%	80.7%
Median % change Functional Marker Subsets	8.36%	8.03%	12.94%
% of population passing	80.4%	79.5%	72.1%

This table illustrates the stability of the assay, showing that the median % change for phenotypic cell populations and functional marker subsets remains within acceptable limits even after accelerated aging for 12 and 24 months.

Pricing

Starting at $1,250 per specimen: teiko.bio/pricing.

Why Choose Spectral Flow Cytometry?

Spectral flow cytometry takes immune monitoring to the next level:

Rapid speeds: Receive publication-quality results on ~100 samples in as little as ~6 weeks.

Read More, Analyze Deeper: Profile 146 immune cell populations and functional marker subsets with unparalleled resolution, covering T cells, B cells, NK cells, monocytes, dendritic cells, and their subsets, in each whole blood sample. You can theoretically measure up to 280 populations on this panel, allowing for detailed pharmacodynamic assessment.

Better Data Quality: Spectral unmixing minimizes signal spillover, ensuring accurate detection of co-expressed markers.

Easier data comparability: If you know conventional flow, you can interpret spectral flow data easily.

Why Choose TokuKit for Fixed Whole Blood?

Access more clinical sites: No centrifugation or fresh blood processing required—collect, fix, and stabilize at the clinical site in under 20 minutes with our TokuKit.
Unmatched Stability: Samples are stable for up to 24 months at -80°C in our accelerated stability studies, enabling true longitudinal studies and batch normalization.
Overcomes the limitations of banked PBMCs: PBMC lose granulocytes, have higher variability in viability, cost $200-$300 per sample, and require specialized equipment and training.
Proven Performance: Achieves a 0.97+ correlation between live and fixed whole blood population frequencies by spectral flow cytometry.

Our service is ideal for:

Global, multi-site clinical trials that need standardized sample handling.
Longitudinal immune monitoring for pharmacodynamic studies.
Reducing variability and enhancing statistical confidence in your results.

We’re solving the three biggest problems in cytometry on clinical trials: getting a blood sample, measuring hundreds of populations, and making sense of it all.

Contact us today to learn how our fixed whole blood spectral flow panel can transform your studies. For orders and inquiries, visit teiko.bio/contact.