Whole Blood Preservation for Clinical Trials

TokuKit

Preserve immune cell populations and functional marker subsets for months.

The three step, centrifuge-free process used at over 40 global trial sites.

3-Step Preservation

Collect, Fix, and Store

Collect Blood

Collect blood at clinical site & invert the vacutainer 10x.

Fix Sample

Transfer 2 mL of blood to Buffer 1; incubate at room temp for 15 mins.

Store in Freezer

Transfer 4.8 mL of sample to Buffer 2; store at -80°C or ship on dry ice.

TokuKit Stability Data

Mass Cytometry

Preservation data on our 41-Marker Standard Panel

15 Month Stability

Preserve population frequencies and protein expression levels from blood draw for 15 months (and counting).

R > 0.99
Correlation of population frequencies
R = 0.96
Correlation of functional marker subsets
R = 0.95
Correlation of protein expression

Post-fixation Stability

Fixed like fresh by mass cytometry

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

R > 0.99
Correlation of population frequencies
R = 0.92
Correlation of functional marker subsets
R = 0.85
Correlation of protein expression

Fresh versus TokuKit-Fixed Specimens

TokuKit Stability Data

Spectral Flow Cytometry

Preservation data with our 25-Marker Standard Panel

6 Month Stability

Preserve population frequencies and protein expression levels from blood draw for 6 months (and counting).

R > 0.99
Correlation of population frequencies
R = 0.98
Correlation of functional marker subsets
R = 0.86
Correlation of median fluorescence intensity

Post-fixation Stability

Fixed like fresh by spectral flow

Comparable population frequencies and protein expression levels between fresh and TokuKit-fixed blood samples.

R = 0.97
Correlation of population frequencies
R = 0.91
Correlation of functional marker subsets
R = 0.84
Correlation of median fluorescence intensity

Fresh versus TokuKit-Fixed Specimens

TokuKit Stability Data

Don't have access to a -80°C freezer?

If necessary, TokuKit-fixed samples can be stored at 4°C for up to 14 days before transferring to a -80°C freezer.

TokuKit-Fixed Specimens, Storage at -80°C versus 4°C

TokuKit

Documentation

Instruction manuals, safety data sheets, and regulatory documentation.

TokuKit Processing Instructions

TokuKit - EDTA Vacutainer TokuKit - Sodium Heparin Vacutainer

Reagant Safety Data Sheets

SMART TUBE - Stable Lyse SMART TUBE - Stable Store

Regulatory Documentation

Nalgene Tubes - Regulatory Guide Nalgene Tubes - ISO 13485:2016 Certification

See TokuKit in Action

Check our TokuKit demo

TokuKit Stability Data

Mass Cytometry

Preservation data on our 41-Marker Standard Panel