Teiko abstracts accepted at ICCS24 in Seattle

Heading to the International Clinical Cytometry Conference (ICCS) in Seattle this October? Meet us there!

Join us on Monday, October 7, at 4:00 PM for the presentation of two posters assessing normalization methods for CyTOF data and analyzing fixed whole blood samples by high-dimensional spectral cytometry.

“Assessment of Batch Normalization Methods on Mass Cytometry Data”
“Rapid on-site fixation of whole blood samples for high-dimensional spectral flow cytometric analysis”

Abstract #205: Assessment of Batch Normalization Methods on Mass Cytometry Data

Presenter: Dr. Gage Black, PhD, Teiko Bio

Batch effects can impact the quality of large biological datasets, including high-dimensional mass cytometry data. In this study, we tested two batch normalization tools, CytoNorm and cyCombine, on a dataset of 68 PBMC samples from 38 melanoma patients.

Abstract #226: Rapid on-site fixation of whole blood samples for high-dimensional spectral flow cytometric analysis

Presenter: Dr. Justin Jarrell, PhD, Teiko Bio

Around 75% of flow cytometry specimens rely on freshly collected blood, requiring processing within 48 hours. This causes complex logistics and can result in >10% sample loss. Fixing whole blood at clinical sites may solve this by preserving samples for years. We used a 25-marker spectral flow cytometry panel on blood from three healthy donors, comparing fixed to live samples. Fixed samples showed a 97% correlation in immune cell populations and a 90% correlation for activation markers (HLA-DR, CD28, CD38, PD-1). Fixation simplifies shipping while preserving sample integrity for clinical trials.

StandardPlus Release

A new cytometry service for customers interested in adding an antibody to track a specific cell type, pathway, or target on CLIA-validated 41-marker CyTOF panels

The StandardPlus service lets customers add an additional antibody to Teiko’s Standard 41-marker peripheral blood mononuclear cell (PBMC) or whole blood (WB) mass cytometry (CyTOF) panel to detect a specific marker of interest. Customers will receive a marker verification report, sample and gating quality control (QC) reports for each cohort of processed samples, and Base, Core, or Premier analysis and deliverables accessible through Teiko’s web application.

Customers appreciate our Standard CyTOF panels for their comprehensive coverage (800+ immune cell types and subsets), reproducibility (CLIA-validation), and flexible pricing (tiered options). However, drug developers often need to track a specific cell type, target, pathway, or a general marker of interest in their clinical trials that isn’t included in the Standard Panel. And while adding just one additional marker to a panel may sound trivial, it actually can take months of work and considerable ($30,000+) fees.

Now, with Teiko, it’s simple. You pick an antibody, and we verify that antibody on a CLIA-validated backbone. And we deliver in the order of weeks (not months) to verify and create a 42-marker CyTOF panel. Get in touch with us about your specific antibody, and we’ll give you a precise estimate.

Our new StandardPlus service ranges from only $100-$300 more per sample compared to our Standard mass cytometry panel. Reach out to us today for more details about how to customize your panel.

Learn more about our Standard+ offerings

StandardPlus Whole Blood StandardPlus PBMC

Online Ordering for TokuKits Live Today

A simple shopping platform to order sample collection TokuKits for clinical trials

Our TokuKits enable the on-site collection and fixation of whole blood samples from clinical trials, preparing them for analysis by high-dimensional mass cytometry or spectral flow cytometry. However, ordering and tracking specimen collection kits have been challenging due to the reliance on manual emails and spreadsheets. To address this, we are excited to announce the launch of our new online ordering system for TokuKits: teiko.bio/tokukit/.

When an order is placed online, it is immediately registered in Teiko’s Laboratory Information Management System (LIMS). This allows us to promptly contact the trial site that placed the order. We additionally keep the clinical trial manager and study sponsor informed. Now everyone is informed about the kits ordered and shipped to all clinical trial sites.

Why not track with emails and spreadsheets?

It might seem obvious, but ordering and tracking kits through emails and spreadsheets is cumbersome, error-prone, and a major headache. Surprisingly, this is a common practice for ordering kits for clinical trials. Without a standardized process, the back-and-forth to collect information delays order fulfillment, preventing critical sample collections from taking place for the clinical trial.

Problem solved with teiko.bio/tokukit

We’ve built a web-based ordering platform, directly coupled to our LIMS to deliver a customer ordering experience that’s centralized, easy to use, informative, and connected to each site using TokuKits in a clinical trial.

Standard ordering template requiring no back and forth emails.
Clinical trial ID entry to automatically sync all orders to the project.
Site ID entry to enable mult-site kit tracking in an upcoming release.
Outbound notification infrastructure to inform study sponsor, clinical trial manager and site contact.
Inbound notification infrastructure to inform study sponsor and clinical site manager on kit receipt.

Ordering TokuKits on teiko.bio/tokukit

In the gif below, we show how to order kits from teiko.bio/tokukit/. We’ll charge them directly to your sponsor account, no credit card needed. This allows you to place kit orders, and allows us to obtain information about the site, and clinical trials. We then collect shipping information. With this information we send tracking information and share the data with both the person who placed the order, the study sponsor and clinical trial manager.