What is gate tailoring? When and why do you perform tailoring on samples?

We tailor, or adjust, gates between patients because each individual has a unique immune setpoint, meaning their immune cells may express slightly higher or lower levels of various protein markers at baseline. This causes the populations to shift on the plots as you cycle between patients and the gates must be moved to accommodate this shift on a per-patient basis. Once the gates are set for each patient, we do not shift them in between time points or conditions. This ensures we are able to capture shifts in expression of state markers across time and conditions based on each patient’s unique baseline.